Description:

·         Microtitre wells are coated with specific antibodies to VHS virus.

·         The kit enables 48 screening tests in duplicates, including controls.

·         The whole kit may be used at one time, or sequentially for smaller batches of samples.

·         Assay sensitivity (103 TCID50 / 0.1 ml of the examined fluid) is sufficient for the routine examination of field samples.

·         Total assay time: approximately 4 h.

·         Tested by the Veterinary Research Institute, Brno, Czech Republic and the State Veterinary Institutes in the Czech Republic.

·         Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic.

·         The kit is manufactured in accordance with Good Manufacturing Practice (GMP).

·         Shelf life: 18 months.

Applications:

·         Detection of VHSV in organ homogenates and in the media of infected cell cultures.

·         Diagnosis of the infection.

·         Control of fish before transport.

·         Control of quarantined fish (export, import).

Brief assay procedure:

1.       Dilute samples and pipette them into the wells:

a) screening test: 1+1 - dilute the samples directly in the wells.

b) titration test: 1+1 to 1+255 - dilute the samples directly in the wells.

2.       Incubate for 60 min at 37 °C.

3.       Aspirate and wash the wells 3 times.

4.       Add rabbit antibodies to VHSV.

5.       Incubate for 60 min at 37 °C.

6.       Aspirate and wash the wells 3 times.

7.       Add Conjugate (an antibody to rabbit IgG labelled with peroxidase).

8.       Incubate for 60 min at 37 °C.

9.       Aspirate and wash the wells 3 times.

10.   Add substrate (TMB-Complete).

11.   Incubate for 10 min at 20 °C.

12.   Add Stopping solution (H2SO4).

13.   Read absorbance photometrically at 450 nm.

14.   Evaluate results.