Description:
- Microtitre wells are coated with the specific antigens of strain Treponema pallidum, mainly p17, p47, p41, p15.
- If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
- The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
Advantages:
- The total assay time is about 1 hours 15 minutes.
- High sensitivity and specificity of the test.
- Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
- Semi-quantitative evaluation in the Index of Positivity (IP).
- Ready-to-use, color-coded components.
- Single-component substrate.
- Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).
Application:
- Qualitative test for detection of antibodies against Treponema pallidum.
Brief assay procedure:
- Samples are used in undiluted form.
- Pipette controls and undiluted samples.
- Add Conjugate.
- Incubate for 30 min at 37 °C.
- Aspirate and wash the wells 5 times.
- Add substrate (TMB-Complete).
- Incubate for 30 min at 37 °C.
- Add Stopping solution (H2SO4).
- Read photometrically at 450 nm.
- Evaluate results.
SmartEIA kits are specifically designed for automated processing on the Agility® instrument, Dynex Technologies, Inc.