Description:
- Microtitre wells are coated with the antibody to human IgA.
- All the IgA antibodies in the sample, including specific anti-T.gondii antibodies (if present), bind to the anti-human IgA antibody in quantities proportionate to their presence. The specific antibodies bound to the solid phase are subsequently labelled with the Tracer (T. gondii antigen + anti-T.gondii monoclonal antibody to the surface protein p-30 of T. gondii conjugated with horseradish peroxidase) and detected through a colour reaction with substrate (TMB-Complete).
- The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
Advantages:
- The total assay time is about 2 hours 30 minutes.
- High sensitivity and specificity of the test.
- Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
- Semi-quantitative evaluation in the Index of Positivity (IP).
- Ready-to-use, color-coded components.
- Single-component substrate.
- Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).
Application:
- Screening test for the detection of acute infection with Toxoplasma gondii in humans.
- Checking of therapy results using the semiquantitative determination.
Brief assay procedure:
- Dilute samples (1:101).
- Pipette Controls and diluted Samples.
- Incubate at 37°C for 60 min.
- Aspirate and wash the wells 5×
- Pipette Conjugate.
- Incubate at 37°C for 60 min.
- Aspirate and wash the wells 5×
- Pipette Substrate (TMB-Complete).
- Incubate at 37°C for 20 min.
- Pipette Stop Solution.
- Read color intensity at 450 nm.
- Evaluate the test.
SmartEIA kits are specifically designed for automated processing on the Agility® instrument, Dynex Technologies, Inc.