Description:
- Microtitre wells are coated with the purified and inactivated antigen isolated from AD169 strain of Cytomegalovirus.
- If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
- The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.
Advantages:
- The total assay time is about 2 hours.
- High sensitivity and specificity of the test.
- Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators
- Semi-quantitative evaluation in the Index of Positivity (IP)
- Sample diluent contains IgG/RF sorbent.
- Ready-to-use, color-coded components
- Single-component substrate
- Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate)
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Sample diluent contains IgG/RF sorbent.
Application:
- Screening test for the detection of specific IgM antibodies in human serum or plasma.
- Checking of therapy results using the semiquantitative detection.
- Disease phase diagnostics.
Brief assay procedure:
- Dilute samples (1:101).
- Pipette Controls and diluted Samples.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×.
- Pipette Conjugate.
- Incubate at 37°C for 30 min.
- Aspirate and wash the wells 5×.
- Pipette Substrate (TMB-Complete).
- Incubate at 37°C for 15 min.
- Pipette Stop Solution.
- Read color intensity at 450 nm
- Evaluate the test.
SmartEIA kits are specifically designed for automated processing on the Agility® instrument, Dynex Technologies, Inc.