·         Microtitre wells are coated with highly purified toxin of Bordetella pertussis.

·         If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).

·         The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Advantages:

·         The total assay time is about 2 hours.

·         High sensitivity and specificity of the test.

·         Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (1, 40, 100, 350　IU/ml).

·         Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in AU/ml.

·         Ready-to-use, color-coded components.

·         Single-component substrate.

·         Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).

Application:

·         Diagnostics of post-infection and post-vaccination antibody response.

·         Serological determination of antibodies to pertussis toxin.

Brief assay procedure:

1.       Dilute samples (1:101).

2.       Pipette Controls and diluted Samples.

3.       Incubate at 37°C for 30 min.

4.       Aspirate and wash the wells 5×

5.       Pipette Conjugate.

6.       Incubate at 37°C for 30 min.

7.       Aspirate and wash the wells 5×

8.       Pipette Substrate (TMB-Complete).

9.       Incubate at 37°C for 30 min.

10.   Pipette Stop Solution.

11.   Read color intensity at 450 nm.