Description:

·         Microtitre wells are coated with the specific antibodies to IPN virus.

·         The kit enables 96 screening tests, including controls.

·         The whole kit may be used at one time or sequentially for smaller batches of samples.

·         Assay sensitivity (102 TCID50/ 0.1 ml of the examined fluid) is sufficient for the routine examination of samples.

·         Total time of survey according to sample incubation duration: a) over night: results in second day. b) rapid test: approximately 2.5 h

·         Tested by the Veterinary Research Institute, Brno, Czech Republic and the State Veterinary Institutes in the Czech Republic.

·         Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic.

·         The kit is manufactured in accordance with Good Manufacturing Practice (GMP).

·         Shelf life: 18 months.

Application:

·         Detection of IPNV in organ homogenates and in the media of infected cell cultures.

·         Diagnosis of the infection.

·         Control of fish before transport.

·         Control of quarantined fish (export, import).

Brief assay procedure:

1.       Dilute and pipette samples, control antigens and RAIPNV into the wells:

a) screening test: 1+1 (sample + RAIPNV) directly in the wells.

b) titration test: 1+1 (sample + RAIPNV) to 1+255, directly in the wells.

2.       Incubate:

a) over night: 14-18 hours at 4-8°C

b) rapid test: 60 min at 37°C

3.       Aspirate and wash the wells 4 times.

4.       Add Conjugate (an antibody to rabbit IgG labelled with peroxidase).

5.       Incubate for 60 min at 37 °C.

6.       Aspirate and wash the wells 4 times.

7.       Add substrate (TMB-Complete).

8.       Incubate for 10 min at room temperature (20 °C).

9.       Add Stopping solution (H2SO4).

10.   Read absorbance photometrically at 450 nm.

11.   Evaluate results.