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Home Products Infectious Serology BlueBLOT-LINE Helicobacter IgG

BlueBLOT-LINE Helicobacter IgG

BlueBLOT-LINE Helicobacter IgG
EAN Code: 8595635307805
Catalog number: BD-HpGL24
Package size: 24 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoblot assay is intended for the diagnosis of Helicobacter pylori infection using IgG antibodies in human serum or plasma in the general population. The semi-quantitative automated assay is designed for professional use in a laboratory.

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Description

Description

·         Each single test contains all the reagents (ready-to-use) necessary for performing the test

·         Antigen used: combination of selected parts of the specific antigens of H. pylori (CagA, VacA, UreA, NAP, HpaA, HcpC, GroEL)

·         Recombinant antigens are transferred to the nitrocellulose membrane, if specific antibodies are present in the sample, they will bind to the respective antigens – the complex is labelled with Conjugate and detected through a color reaction with substrate

·         Short incubation periods, total assay time: approximately 1.25 h.

·         Evaluation using Immunoblot Software (TestLine)

 

Applications

·         Diagnostics of H. pylori

·         Confirmation for ELISA tests

 

Brief assay procedure:

1.      Insert testing strips and cartridges into the BlueDiver Instrument.

2.      Pipette samples.

3.      Fully automated incubation and washing steps in BlueDiver Instrument

4.      Incubation 30 minutes.

5.      Wash 3 x 2 min.

6.      Conjugate dispensing.

7.      Incubation 20 min.

8.      Wash 3 x 2 min.

9.      Substrate dispensing.

10.   Incubation 10 min.

11.   Wash 1x 2 min.

12.   Evaluation of test results using the BlueScan scanner and Immunoblot Software.

 
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