Description
· Each single test contains all the reagents (ready-to-use) necessary for performing the test
· Antigen used: combination of selected parts of the specific antigens of H. pylori (CagA, VacA, UreA, NAP, HpaA, HcpC, GroEL)
· Recombinant antigens are transferred to the nitrocellulose membrane, if specific antibodies are present in the sample, they will bind to the respective antigens – the complex is labelled with Conjugate and detected through a color reaction with substrate
· Short incubation periods, total assay time: approximately 1.25 h.
· Evaluation using Immunoblot Software (TestLine)
Applications
· Diagnostics of H. pylori
· Confirmation for ELISA tests
Brief assay procedure:
1. Insert testing strips and cartridges into the BlueDiver Instrument.
2. Pipette samples.
3. Fully automated incubation and washing steps in BlueDiver Instrument
4. Incubation 30 minutes.
5. Wash 3 x 2 min.
6. Conjugate dispensing.
7. Incubation 20 min.
8. Wash 3 x 2 min.
9. Substrate dispensing.
10. Incubation 10 min.
11. Wash 1x 2 min.
12. Evaluation of test results using the BlueScan scanner and Immunoblot Software.