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Home Products Infectious Serology BLOT-LINE Yersinia IgM

BLOT-LINE Yersinia IgM

BLOT-LINE Yersinia IgM
EAN Code: 8595635307256
Catalog number: YML020
Package size: 20 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoblot assay is intended for the diagnosis of Yersinia spp. infection using IgM antibodies in human serum or plasma in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

·         Antigen used: combination of selected parts of the specific antigens of Yersinia spp. (YopB, YopD, YopE, YopH, YopM, YopN, LcrV, Ail, Invasin ) and  species-specific antigens of Y.enterocolitica (YstB, YscM-Y.Ent) and Y.pseudotuberculosis (YscM – Y.Pst).

·         Recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT‑LINE (BL) strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens.

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 20 tests.

·         Short incubation periods, total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).

·         Positive and Negative controls included enabling validation of the tests.

·         BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.

·         Colour identification in compliance with antibody classes.

·         Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT-LINE kits.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Applications:

·         Diagnostics of Yersinia.

·         Confirmatory test to ELISA.

Brief assay procedure:

1.       Dilute serum/plasma samples (1:51).

2.       Pipette diluted samples and controls into the channels of the tray and insert the BL strips.

3.       Incubate for 30 min at room temperature using a shaking apparatus.

4.       Wash 3 times for 5 min.

5.       Add Conjugate.

6.       Incubate for 30 min at room temperature using a shaking apparatus.

7.       Wash 3 times for 5 min.

8.       Add substrate (BCIP/NBT).

9.       Incubate for 15 min at room temperature using a shaking apparatus.

10.   Wash 2 times for 5 min in distilled water.

11.   Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.

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