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Home Products Infectious Serology BLOT-LINE Borrelia/HGA IgG

BLOT-LINE Borrelia/HGA IgG

BLOT-LINE Borrelia/HGA IgG
EAN Code: 8595635300721
Catalog number: BGL020
Package size: 20 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoblot assay is intended for the diagnosis of Borrelia spp. and Anaplasma phagocytophilum infections using IgG antibodies in human serum, plasma, cerebrospinal fluid or synovial fluid in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

·         Antigen used: combination of selected parts of the specific antigens of Borrelia sp. (VlsE B. afzelii, VlsE B. garinii, VlsE B. b. sensu stricto, p83, p41, p39, OspB, OspA B.afzelii, OspA B.garinii, OspA B.b. sensu stricto, OspC a p17), high specific antigen of Anaplasma phagocytophilum (p44) and recombinant antigen TpN17 for exclusion of cross-reactivity with Treponema pallidum.

·         Recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT‑LINE (BL) strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens.

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 20 tests.

·         Short incubation periods, total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).

·         BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.

·         Positive and Negative controls included enabling validation of the tests.

·         Colour identification in compliance with antibody classes.

·         Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT and BLOT-LINE Borrelia kits.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Applications:

·         Laboratory test for the detection of Lyme borreliosis, confirmatory test to ELISA.

·         Laboratory test for the detection of Anaplasmosis.

Brief assay procedure:

1.       Dilute serum/plasma samples (1:51), cerebrospinal fluids (1:2), synovial fluid (1:17.5).

2.       Pipette diluted samples and controls into the channels of the tray and insert the BL strips.

3.       Incubate for 30 min at room temperature using a shaking apparatus.

4.       Wash 3 times for 5 min.

5.       Add Conjugate.

6.       Incubate for 30 min at room temperature using a shaking apparatus.

7.       Wash 3 times for 5 min.

8.       Add substrate (BCIP/NBT).

9.       Incubate for 15 min at room temperature using a shaking apparatus.

10.   Wash 2 times for 5 min in distilled water.

11.   Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.

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