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Home Products Infectious Serology BLOT-LINE Borrelia garinii IgM

BLOT-LINE Borrelia garinii IgM

BLOT-LINE Borrelia garinii IgM
EAN Code: 8595635301063
Catalog number: BgML20
Package size: 20 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoblot assay is intended for the diagnosis of Borrelia garinii infection using IgM antibodies in human serum, plasma or cerebrospinal fluid in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

·         Antigen used: combination of selected parts of the specific antigens of Borrelia garinii (VlsE, p83, p41, p39 and OspC).

·         Recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT‑LINE (BL) strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens.

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 20 tests.

·         Short incubation periods, total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).

·         Positive and Negative controls included enabling validation of the tests.

·         BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.

·         Colour identification in compliance with antibody classes.

·        Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT-LINE kits.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Applications:

·         Laboratory test for the detection of Lyme borreliosis, confirmatory test to ELISA.

Brief assay procedure:

1.       Dilute serum/plasma samples (1:51), cerebrospinal fluids (1:2)

2.       Pipette diluted samples and controls into the channels of the tray and insert the BL strips.

3.       Incubate for 30 min at room temperature using a shaking apparatus.

4.       Wash 3 times for 5 min.

5.       Add Conjugate.

6.       Incubate for 30 min at room temperature using a shaking apparatus.

7.       Wash 3 times for 5 min.

8.       Add substrate (BCIP/NBT).

9.       Incubate for 15 min at room temperature using a shaking apparatus.

10.   Wash 2 times for 5 min in distilled water.

11.   Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.

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