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Home Products Infectious Serology BLOT-LINE Bordetella IgG

BLOT-LINE Bordetella IgG

BLOT-LINE Bordetella IgG
EAN Code: 8595635304453
Catalog number: BpGL20
Package size: 20 tests
Regulatory status: IVD CE
Storage: 2-8 °C
Producer: TestLine Clinical Diagnostics s.r.o.

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Contact person:

Name: MUDr. Jana Dočkalová
Phone: +420 549 121 205
E-mail: trade@testlinecd.com

The immunoblot assay is intended for the diagnosis of Bordetella pertussis and Bordetella parapertussis infection using IgG antibodies in human serum or plasma in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.

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Description

Description:

·         Antigen used: combination of selected parts of the specific antigens of Bordetella (B. pertussis - pertussis toxin (PT), filamentous hemagglutinin (FHA), adenylate cyclase toxin (ACT), tracheal colonization factor (TCF), B. parapertussis – outer membrane protein (Pertactin), adhesion (FimN), entericidin A (EntA).

·         Recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT‑LINE (BL) strips).

·         If specific antibodies are present in the sample, they will bind to the respective antigens.

·         The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).

·         The kit enables 20 tests.

·         Short incubation periods, total assay time: approximately 1.5 h.

·         High sensitivity and specificity.

·         All reagents supplied are ready to use.

·         The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).

·         Positive and Negative controls included enabling validation of the tests.

·         BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.

·         Colour identification in compliance with antibody classes.

·         Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT-LINE kits.

·         Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Applications:

·         Laboratory test for the detection of B. pertusis and B. parapertussis, confirmatory test to ELISA.

·         Laboratory test for the detection of B. pertusis and B. parapertussis.

Brief assay procedure:

1.       Dilute serum/plasma samples (1:51).

2.       Pipette diluted samples and controls into the channels of the tray and insert the BL strips.

3.       Incubate for 30 min at room temperature using a shaking apparatus.

4.       Wash 3 times for 5 min.

5.       Add Conjugate.

6.       Incubate for 30 min at room temperature using a shaking apparatus.

7.       Wash 3 times for 5 min.

8.       Add substrate (BCIP/NBT).

9.       Incubate for 15 min at room temperature using a shaking apparatus.

10.   Wash 2 times for 5 min in distilled water.

11.   Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.

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