Description:

·         The kit is standardized according to the OIE International Standard Serum EU2.

·         The kit enables 480 screening tests, including controls.

·         The whole kit may be used at one time or sequentially for smaller batches of samples.

·         Microtitre wells are coated with the purified and inactivated BHV-1 antigen.

·         Total assay time: approximately 2 h.

·         The evaluation is based on ratio of the absorbance of the sample to the absorbance of the positive control serum-CUT OFF expressed in percentage (%).

·         Tested by the Veterinary Research Institute, Brno, Czech Republic and State Veterinary Institutes in the Czech Republic.

·         Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic.

·         The kit is manufactured in accordance with Good Manufacturing Practice (GMP).

·         Shelf life: 15 months.

Application:

·         Screening of IBR occurrence in a cattle population.

·         Identification of infected or vaccinated animals, checking of vaccination effectiveness.

·         Obtaining the background information to formulate sanitation programmes for cattle herds or regions, control of current sanitation process in cattle herds.

·         Evaluation of effects of the sanitation programme, ongoing screening in herds after sanitation.

·         Control of transported and quarantined animals (export, import).

Brief assay procedure:

1.       Dilute samples and controls (1:20) in microplate wells.

2.       Incubate for 60 min at 37 °C.

3.       Aspirate and wash the wells 4 times.

4.       Add Conjugate.

5.       Incubate for 30 min at 37 °C.

6.       Aspirate and wash the wells 4 times.

7.       Add substrate (TMB-Complete).

8.       Incubate for 15 min at room temperature.

9.       Add Stopping solution (H2SO4).

10.   Read absorbance photometrically at 450 nm.

11.   Evaluate results.