Description:

·         The kit is standardized according to the OIE International Reference Standard Serum (Community reference serum ADV1).

·         The microtitre plate is coated with inactivated and purified suid herpesvirus 1 antigen.

·         The kit enables 480 screening tests, including controls.

·         The whole kit may be used sequentially for smaller batches of samples.

·         Microtitre wells are coated with the viral antigens.

·         ELISA is more sensitive than the virus neutralisation test (VNT) using the 100 TCID50.

·         Total assay time: approximately 1,5 h.

·         Tested at the Veterinary Research Institute, Brno, Czech Republic and State Veterinary Institutes in the Czech Republic.

·         Approved by the Institute for State Control of Veterinary Biologicals and Medicaments, Czech Republic.

·         The kit is manufactured in accordance with Good Manufacturing Practice (GMP).

·         Shelf life: 24 months.

Application:

·         Screening of Aujeszky's disease occurrence in swine herds.

·         Diagnosis of the infection.

·         Checking of vaccination effectiveness.

·         Control of transported and quarantined animals (export, import).

·         Determination of antibodies in samples of imported unprocessed meat.

Brief assay procedure:

1.       Dilute samples and pipette them into the wells (1:20).

2.       Incubate for 30 min at 37 °C.

3.       Aspirate and wash the wells 4 times.

4.       Add Conjugate.

5.       Incubate for 30 min at 37 °C.

6.       Aspirate and wash the wells 4 times.

7.       Add substrate (TMB-Complete).

8.       Incubate for 15 min at room temperature.

9.       Add Stopping solution (H2SO4).

10.   Read the absorbance photometrically at 450 nm.

11.   Evaluate results.